Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning

Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning

Summary: Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be ident...

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Bibliographic Details
Main Authors: Cyrus M. Haas, Irene M. Francino-Urdaniz, Paul J. Steiner, Timothy A. Whitehead
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Sequence analysis
Sequencing
Immunology
Microbiology
Molecular Biology
Antibody
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100575X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672100575X

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