Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis
Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from c...
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Bogor Agricultural University
2012-06-01
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doaj-d350c4b968d9485a9a69ad175ecee7492020-11-24T21:41:21ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942012-06-011929398Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer AnalysisCECILIA ANNA SEUMAHUANTONIUS SUWANTOIMAN RUSMANADEDY DURYADI SOLIHINTempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile.http://journal.ipb.ac.id/index.php/hayati/article/view/5310/3706ARISA profileDNA extractiontempe microbial community |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
CECILIA ANNA SEUMAHU ANTONIUS SUWANTO IMAN RUSMANA DEDY DURYADI SOLIHIN |
spellingShingle |
CECILIA ANNA SEUMAHU ANTONIUS SUWANTO IMAN RUSMANA DEDY DURYADI SOLIHIN Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis Hayati Journal of Biosciences ARISA profile DNA extraction tempe microbial community |
author_facet |
CECILIA ANNA SEUMAHU ANTONIUS SUWANTO IMAN RUSMANA DEDY DURYADI SOLIHIN |
author_sort |
CECILIA ANNA SEUMAHU |
title |
Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis |
title_short |
Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis |
title_full |
Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis |
title_fullStr |
Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis |
title_full_unstemmed |
Comparison of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer Analysis |
title_sort |
comparison of dna extraction methods for microbial community analysis in indonesian tempe employing amplified ribosomal intergenic spacer analysis |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 2086-4094 |
publishDate |
2012-06-01 |
description |
Tempe fermentation involved complex microbial communities which are only revealed partially through culture dependent methods. Culture-independent methods would be potential to unravel this complex microbial fermentation. Appropriate DNA extraction is an essential tool to obtain reliable data from culture independent method. In this study, we employed two commercial DNA extraction methods to find the best one for microbial community characterization employing amplified ribosomal intergenic spacer analysis (ARISA). Our result showed that PowerFood Microbial DNA Isolation Kit-MOBIO (PFMDIK) is an excellent method for microbial DNA extraction from tempe. It gave high quantity and quality of DNA suitable for PCR amplification of 16S-23S rRNA intergenic spacer to yield a diverse and reproducible ARISA profile. |
topic |
ARISA profile DNA extraction tempe microbial community |
url |
http://journal.ipb.ac.id/index.php/hayati/article/view/5310/3706 |
work_keys_str_mv |
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