Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants
Objectives: In 2014 probiotic supplementation (Lactobacillus acidophilus and Bifidobacterium longum subspecies infantis; InfloranⓇ) was introduced as standard of care to prevent necrotizing enterocolitis (NEC) in extremely preterm infants in Norway. We aimed to evaluate the influence of probiotics a...
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doaj-d3994ba4168740bcbdcf84ee5334e8b52020-11-24T23:06:47ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602018-11-01610.3389/fped.2018.00347411031Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm InfantsEirin Esaiassen0Eirin Esaiassen1Erik Hjerde2Jorunn Pauline Cavanagh3Jorunn Pauline Cavanagh4Tanja Pedersen5Jannicke H. Andresen6Siren I. Rettedal7Ragnhild Støen8Ragnhild Støen9Britt Nakstad10Britt Nakstad11Nils P. Willassen12Claus Klingenberg13Claus Klingenberg14Paediatric Research Group, Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø, NorwayDepartment of Paediatrics, University Hospital of North Norway, Tromsø, NorwayDepartment of Chemistry, Norstruct, UiT The Arctic University of Norway, Tromsø, NorwayPaediatric Research Group, Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø, NorwayDepartment of Paediatrics, University Hospital of North Norway, Tromsø, NorwayDepartment of Paediatrics, Haukeland University Hospital, Bergen, NorwayDepartment of Neonatal Intensive Care, Oslo University Hospital, Oslo, NorwayDepartment of Paediatrics, Stavanger University Hospital, Stavanger, NorwayDepartment of Paediatrics, St. Olavs University Hospital, Trondheim, NorwayDepartment of Laboratory Medicine, Children‘s and Women‘s Health, University of Science and Technology, Trondheim, NorwayDepartment of Paediatric and Adolescents Medicine, Akershus University Hospital, Nordbyhagen, Norway0Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, NorwayDepartment of Chemistry, Norstruct, UiT The Arctic University of Norway, Tromsø, NorwayPaediatric Research Group, Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø, NorwayDepartment of Paediatrics, University Hospital of North Norway, Tromsø, NorwayObjectives: In 2014 probiotic supplementation (Lactobacillus acidophilus and Bifidobacterium longum subspecies infantis; InfloranⓇ) was introduced as standard of care to prevent necrotizing enterocolitis (NEC) in extremely preterm infants in Norway. We aimed to evaluate the influence of probiotics and antibiotic therapy on the developing gut microbiota and antibiotic resistome in extremely preterm infants, and to compare with very preterm infants and term infants not given probiotics.Study design: A prospective, observational multicenter study in six tertiary-care neonatal units. We enrolled 76 infants; 31 probiotic-supplemented extremely preterm infants <28 weeks gestation, 35 very preterm infants 28–31 weeks gestation not given probiotics and 10 healthy full-term control infants. Taxonomic composition and collection of antibiotic resistance genes (resistome) in fecal samples, collected at 7 and 28 days and 4 months age, were analyzed using shotgun-metagenome sequencing.Results: Median (IQR) birth weight was 835 (680–945) g and 1,290 (1,150–1,445) g in preterm infants exposed and not exposed to probiotics, respectively. Two extremely preterm infants receiving probiotic developed NEC requiring surgery. At 7 days of age we found higher median relative abundance of Bifidobacterium in probiotic supplemented infants (64.7%) compared to non-supplemented preterm infants (0.0%) and term control infants (43.9%). Lactobacillus was only detected in small amounts in all groups, but the relative abundance increased up to 4 months. Extremely preterm infants receiving probiotics had also much higher antibiotic exposure, still overall microbial diversity and resistome was not different than in more mature infants at 4 weeks and 4 months.Conclusion: Probiotic supplementation may induce colonization resistance and alleviate harmful effects of antibiotics on the gut microbiota and antibiotic resistome.Clinical Trial Registration: Clinicaltrials.gov: NCT02197468. https://clinicaltrials.gov/ct2/show/NCT02197468https://www.frontiersin.org/article/10.3389/fped.2018.00347/fullgut microbiotapreterm infantshotgun metagenome sequencingtaxonomybifidobacterialactobacilli |
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English |
format |
Article |
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DOAJ |
author |
Eirin Esaiassen Eirin Esaiassen Erik Hjerde Jorunn Pauline Cavanagh Jorunn Pauline Cavanagh Tanja Pedersen Jannicke H. Andresen Siren I. Rettedal Ragnhild Støen Ragnhild Støen Britt Nakstad Britt Nakstad Nils P. Willassen Claus Klingenberg Claus Klingenberg |
spellingShingle |
Eirin Esaiassen Eirin Esaiassen Erik Hjerde Jorunn Pauline Cavanagh Jorunn Pauline Cavanagh Tanja Pedersen Jannicke H. Andresen Siren I. Rettedal Ragnhild Støen Ragnhild Støen Britt Nakstad Britt Nakstad Nils P. Willassen Claus Klingenberg Claus Klingenberg Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants Frontiers in Pediatrics gut microbiota preterm infant shotgun metagenome sequencing taxonomy bifidobacteria lactobacilli |
author_facet |
Eirin Esaiassen Eirin Esaiassen Erik Hjerde Jorunn Pauline Cavanagh Jorunn Pauline Cavanagh Tanja Pedersen Jannicke H. Andresen Siren I. Rettedal Ragnhild Støen Ragnhild Støen Britt Nakstad Britt Nakstad Nils P. Willassen Claus Klingenberg Claus Klingenberg |
author_sort |
Eirin Esaiassen |
title |
Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants |
title_short |
Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants |
title_full |
Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants |
title_fullStr |
Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants |
title_full_unstemmed |
Effects of Probiotic Supplementation on the Gut Microbiota and Antibiotic Resistome Development in Preterm Infants |
title_sort |
effects of probiotic supplementation on the gut microbiota and antibiotic resistome development in preterm infants |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pediatrics |
issn |
2296-2360 |
publishDate |
2018-11-01 |
description |
Objectives: In 2014 probiotic supplementation (Lactobacillus acidophilus and Bifidobacterium longum subspecies infantis; InfloranⓇ) was introduced as standard of care to prevent necrotizing enterocolitis (NEC) in extremely preterm infants in Norway. We aimed to evaluate the influence of probiotics and antibiotic therapy on the developing gut microbiota and antibiotic resistome in extremely preterm infants, and to compare with very preterm infants and term infants not given probiotics.Study design: A prospective, observational multicenter study in six tertiary-care neonatal units. We enrolled 76 infants; 31 probiotic-supplemented extremely preterm infants <28 weeks gestation, 35 very preterm infants 28–31 weeks gestation not given probiotics and 10 healthy full-term control infants. Taxonomic composition and collection of antibiotic resistance genes (resistome) in fecal samples, collected at 7 and 28 days and 4 months age, were analyzed using shotgun-metagenome sequencing.Results: Median (IQR) birth weight was 835 (680–945) g and 1,290 (1,150–1,445) g in preterm infants exposed and not exposed to probiotics, respectively. Two extremely preterm infants receiving probiotic developed NEC requiring surgery. At 7 days of age we found higher median relative abundance of Bifidobacterium in probiotic supplemented infants (64.7%) compared to non-supplemented preterm infants (0.0%) and term control infants (43.9%). Lactobacillus was only detected in small amounts in all groups, but the relative abundance increased up to 4 months. Extremely preterm infants receiving probiotics had also much higher antibiotic exposure, still overall microbial diversity and resistome was not different than in more mature infants at 4 weeks and 4 months.Conclusion: Probiotic supplementation may induce colonization resistance and alleviate harmful effects of antibiotics on the gut microbiota and antibiotic resistome.Clinical Trial Registration: Clinicaltrials.gov: NCT02197468. https://clinicaltrials.gov/ct2/show/NCT02197468 |
topic |
gut microbiota preterm infant shotgun metagenome sequencing taxonomy bifidobacteria lactobacilli |
url |
https://www.frontiersin.org/article/10.3389/fped.2018.00347/full |
work_keys_str_mv |
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