Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo
Few treatment options exist for metastatic prostate cancer (PC) that becomes hormone refractory (HRPC). In vitro, plant-derived MINA-05 caused dose-dependent decreases in cell numbers in HRPC cell lines LNCaPC4-2B and PC-3, and in androgen-sensitive LNCaP-FGC, DuCaP, and LAPC-4, by WST-1 assay. MIN...
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doaj-d3b383c86af24a0582ad94cf736057d02020-11-25T00:15:30ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022007-04-019432233110.1593/neo.06775Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In VivoKate Vandyke0Melanie Y. White1Terry Nguyen-Khuong2Kim Ow3Sharon C.-W. Luk4Elizabeth A. Kingsley5Alexandra Rowe6Shiu-Fun Pang7Bradley J. Walsh8Pamela J. Russell9Oncology Research Centre, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, AustraliaMinomic Pty Ltd, PO Box 664, Harbord, NSW 2096, AustraliaMinomic Pty Ltd, PO Box 664, Harbord, NSW 2096, AustraliaOncology Research Centre, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, AustraliaCK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong KongOncology Research Centre, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, AustraliaOncology Research Centre, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, AustraliaCK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong KongMinomic Pty Ltd, PO Box 664, Harbord, NSW 2096, AustraliaOncology Research Centre, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, Australia Few treatment options exist for metastatic prostate cancer (PC) that becomes hormone refractory (HRPC). In vitro, plant-derived MINA-05 caused dose-dependent decreases in cell numbers in HRPC cell lines LNCaPC4-2B and PC-3, and in androgen-sensitive LNCaP-FGC, DuCaP, and LAPC-4, by WST-1 assay. MINA-05 pretreatment significantly decreased clonogenic survival in agar and on plastic at 1 × and 2 × IC50 for PC-3 (P < .05 and P < .001, respectively), and at 1/2 ×, 1 ×, and 2 × IC50 for LNCaP-FGC cells (P < .001). MINA-05 also induced G2M arrest of LNCaP-FGC and PC-3 cells (by flow cytometry) and caused some apoptosis in LNCaPFGC (sub-G1, peak on flow, expression of activated caspase-3) but not in PC-3 cells. Western blotting indicated that these cell cycle changes were associated with decreased levels of regulatory proteins cyclin B1 and cdc25C. MINA-05 given daily by gavage for 39 days did not diminish primary orthotopic PC-3 growth in nude mice, but decreased the extent of lymph node invasion at higher doses. We conclude that MINA-05 induces G2M arrest, inhibits cell growth, reduces PC cell re-growth in vitro, and reduces lymph node invasion after orthotopic PC-3 cell implantation in vivo. It has potential as an adjuvant treatment for patients with PC. http://www.sciencedirect.com/science/article/pii/S1476558607801023Prostate cancerG2M blockMINA-05 herbal treatmentlymph node metastasesclonogenic assays |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kate Vandyke Melanie Y. White Terry Nguyen-Khuong Kim Ow Sharon C.-W. Luk Elizabeth A. Kingsley Alexandra Rowe Shiu-Fun Pang Bradley J. Walsh Pamela J. Russell |
spellingShingle |
Kate Vandyke Melanie Y. White Terry Nguyen-Khuong Kim Ow Sharon C.-W. Luk Elizabeth A. Kingsley Alexandra Rowe Shiu-Fun Pang Bradley J. Walsh Pamela J. Russell Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo Neoplasia: An International Journal for Oncology Research Prostate cancer G2M block MINA-05 herbal treatment lymph node metastases clonogenic assays |
author_facet |
Kate Vandyke Melanie Y. White Terry Nguyen-Khuong Kim Ow Sharon C.-W. Luk Elizabeth A. Kingsley Alexandra Rowe Shiu-Fun Pang Bradley J. Walsh Pamela J. Russell |
author_sort |
Kate Vandyke |
title |
Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo |
title_short |
Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo |
title_full |
Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo |
title_fullStr |
Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo |
title_full_unstemmed |
Plant-Derived MINA-05 Inhibits Human Prostate Cancer Proliferation In Vitro and Lymph Node Spread In Vivo |
title_sort |
plant-derived mina-05 inhibits human prostate cancer proliferation in vitro and lymph node spread in vivo |
publisher |
Elsevier |
series |
Neoplasia: An International Journal for Oncology Research |
issn |
1476-5586 1522-8002 |
publishDate |
2007-04-01 |
description |
Few treatment options exist for metastatic prostate cancer (PC) that becomes hormone refractory (HRPC). In vitro, plant-derived MINA-05 caused dose-dependent decreases in cell numbers in HRPC cell lines LNCaPC4-2B and PC-3, and in androgen-sensitive LNCaP-FGC, DuCaP, and LAPC-4, by WST-1 assay. MINA-05 pretreatment significantly decreased clonogenic survival in agar and on plastic at 1 × and 2 × IC50 for PC-3 (P < .05 and P < .001, respectively), and at 1/2 ×, 1 ×, and 2 × IC50 for LNCaP-FGC cells (P < .001). MINA-05 also induced G2M arrest of LNCaP-FGC and PC-3 cells (by flow cytometry) and caused some apoptosis in LNCaPFGC (sub-G1, peak on flow, expression of activated caspase-3) but not in PC-3 cells. Western blotting indicated that these cell cycle changes were associated with decreased levels of regulatory proteins cyclin B1 and cdc25C. MINA-05 given daily by gavage for 39 days did not diminish primary orthotopic PC-3 growth in nude mice, but decreased the extent of lymph node invasion at higher doses. We conclude that MINA-05 induces G2M arrest, inhibits cell growth, reduces PC cell re-growth in vitro, and reduces lymph node invasion after orthotopic PC-3 cell implantation in vivo. It has potential as an adjuvant treatment for patients with PC.
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topic |
Prostate cancer G2M block MINA-05 herbal treatment lymph node metastases clonogenic assays |
url |
http://www.sciencedirect.com/science/article/pii/S1476558607801023 |
work_keys_str_mv |
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