Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions

Measuring growth factors in single cells at physiologically relevant stimulation doses is challenging. Here the authors use fluorescent quantum dots and calibrated three-dimensional deconvolution microscopy to digitally count growth factors in single cells and reveal stimulation distributions in can...

Full description

Bibliographic Details
Main Authors: Phuong Le, Sung Jun Lim, Brian C. Baculis, Hee Jung Chung, Kristopher A. Kilian, Andrew M. Smith
Format: Article
Language:English
Published: Nature Publishing Group 2019-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-08754-5