Characterization of 16 novel microsatellite loci for Ephippiger diurnus (Orthoptera: Tettigoniidae) using pyrosequencing technology and cross-species amplification

A novel panel of 16 microsatellite markers, obtained by pyrosequencing of enriched genomic libraries, is reported for the flightless European bushcricket Ephippiger diurnus (Dufour) (Orthoptera: Tettigoniidae). Five multiplex and one simplex PCR protocols were optimized, and the polymorphism at the...

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Bibliographic Details
Main Authors: Yareli ESQUER-GARRIGOS, Michael D. GREENFIELD, Virginie PARTY, Réjane STREIFF
Format: Article
Language:English
Published: Institute of Entomology, Biology Centre, Czech Academy of Science 2016-04-01
Series:European Journal of Entomology
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Online Access:https://www.eje.cz/artkey/eje-201601-0037_Characterization_of_16_novel_microsatellite_loci_for_Ephippiger_diurnus_Orthoptera_Tettigoniidae_using_pyros.php