Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals β4-Mediated Control of Channel Clustering in Cerebellum

Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals β4-Mediated Control of Channel Clustering in Cerebellum

BK channels are critical regulators of neuronal activity, controlling firing, neurotransmitter release, cerebellar function, and BK channel mutations have been linked to seizure disorders. Modulation of BK channel gating is well characterized, regulated by accessory subunit interactions, intracellul...

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Bibliographic Details
Main Authors: Christopher P. Pratt, Dika A. Kuljis, Gregg E. Homanics, Jianjun He, Dmytro Kolodieznyi, Srikanth Dudem, Mark A. Hollywood, Alison L. Barth, Marcel P. Bruchez
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-10-01
Series:Frontiers in Cellular Neuroscience
Subjects:
BK channels
CRISPR/Cas9
fluorogen-activating peptides
knock-in
Purkinje cells
fluorescent dyes
Online Access:http://journal.frontiersin.org/article/10.3389/fncel.2017.00337/full

Internet

http://journal.frontiersin.org/article/10.3389/fncel.2017.00337/full

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