Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation

Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation

Adaptive immunity of prokaryotes depends on acquisition of foreign DNA fragments into CRISPR arrays as spacers followed by destruction of foreign DNA by CRISPR interference machinery. Different fragments are acquired into CRISPR arrays with widely different efficiencies, but the factors responsible...

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Bibliographic Details
Main Authors: Olga Musharova, Danylo Vyhovskyi, Sofia Medvedeva, Jelena Guzina, Yulia Zhitnyuk, Marko Djordjevic, Konstantin Severinov, Ekaterina Savitskaya
Format: Article
Language:English
Published: American Society for Microbiology 2018-12-01
Series:mBio
Subjects:
CRISPR spacers
CRISPR-Cas
naïve adaptation
primed adaptation
Online Access:https://doi.org/10.1128/mBio.02169-18

Internet

https://doi.org/10.1128/mBio.02169-18

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