Pooled extracellular receptor-ligand interaction screening using CRISPR activation

Pooled extracellular receptor-ligand interaction screening using CRISPR activation

Abstract Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors fo...

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Bibliographic Details
Main Authors: Zheng-Shan Chong, Shuhei Ohnishi, Kosuke Yusa, Gavin J. Wright
Format: Article
Language:English
Published: BMC 2018-11-01
Series:Genome Biology
Subjects:
Cell surface receptors
Cell signaling
CRISPR activation
Extracellular protein interactions
Flow cytometry
Genome-wide screening
Online Access:http://link.springer.com/article/10.1186/s13059-018-1581-3

Internet

http://link.springer.com/article/10.1186/s13059-018-1581-3

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