MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation.
Development of mammary alveolar epithelium during pregnancy is controlled by prolactin, through the transcription factors STAT5A/B that activate specific sets of target genes. Here we asked whether some of STAT5's functions are mediated by microRNAs. The miR-21 promoter sequence contains a bona...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3907398?pdf=render |
id |
doaj-d43b345da6e8488e99e1ec368fe87a7d |
---|---|
record_format |
Article |
spelling |
doaj-d43b345da6e8488e99e1ec368fe87a7d2020-11-24T20:51:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8512310.1371/journal.pone.0085123MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation.Yonatan FeuermannKeunsoo KangAvi ShamayGertraud W RobinsonLothar HennighausenDevelopment of mammary alveolar epithelium during pregnancy is controlled by prolactin, through the transcription factors STAT5A/B that activate specific sets of target genes. Here we asked whether some of STAT5's functions are mediated by microRNAs. The miR-21 promoter sequence contains a bona-fide STAT5 binding site and miR-21 levels increased in HC11 mammary cells upon prolactin treatment. In vivo miR-21 was abundantly expressed in mammary epithelium at day 6 of pregnancy. Analysis of mice lacking miR-21 revealed that their mammary tissue developed normally during pregnancy and dams were able to nurse their pups. Our study demonstrated that although expression of miR-21 is under prolactin control through the transcription factors STAT5A/B its presence is dispensable for mammary development and lactation.http://europepmc.org/articles/PMC3907398?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yonatan Feuermann Keunsoo Kang Avi Shamay Gertraud W Robinson Lothar Hennighausen |
spellingShingle |
Yonatan Feuermann Keunsoo Kang Avi Shamay Gertraud W Robinson Lothar Hennighausen MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation. PLoS ONE |
author_facet |
Yonatan Feuermann Keunsoo Kang Avi Shamay Gertraud W Robinson Lothar Hennighausen |
author_sort |
Yonatan Feuermann |
title |
MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation. |
title_short |
MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation. |
title_full |
MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation. |
title_fullStr |
MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation. |
title_full_unstemmed |
MiR-21 is under control of STAT5 but is dispensable for mammary development and lactation. |
title_sort |
mir-21 is under control of stat5 but is dispensable for mammary development and lactation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Development of mammary alveolar epithelium during pregnancy is controlled by prolactin, through the transcription factors STAT5A/B that activate specific sets of target genes. Here we asked whether some of STAT5's functions are mediated by microRNAs. The miR-21 promoter sequence contains a bona-fide STAT5 binding site and miR-21 levels increased in HC11 mammary cells upon prolactin treatment. In vivo miR-21 was abundantly expressed in mammary epithelium at day 6 of pregnancy. Analysis of mice lacking miR-21 revealed that their mammary tissue developed normally during pregnancy and dams were able to nurse their pups. Our study demonstrated that although expression of miR-21 is under prolactin control through the transcription factors STAT5A/B its presence is dispensable for mammary development and lactation. |
url |
http://europepmc.org/articles/PMC3907398?pdf=render |
work_keys_str_mv |
AT yonatanfeuermann mir21isundercontrolofstat5butisdispensableformammarydevelopmentandlactation AT keunsookang mir21isundercontrolofstat5butisdispensableformammarydevelopmentandlactation AT avishamay mir21isundercontrolofstat5butisdispensableformammarydevelopmentandlactation AT gertraudwrobinson mir21isundercontrolofstat5butisdispensableformammarydevelopmentandlactation AT lotharhennighausen mir21isundercontrolofstat5butisdispensableformammarydevelopmentandlactation |
_version_ |
1716801228149620736 |