Combining multi-mutant and modular thermodynamic cycles to measure energetic coupling networks in enzyme catalysis
We measured and cross-validated the energetics of networks in Bacillus stearothermophilus Tryptophanyl-tRNA synthetase (TrpRS) using both multi-mutant and modular thermodynamic cycles. Multi-dimensional combinatorial mutagenesis showed that four side chains from this “molecular switch” move coordina...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC and ACA
2017-05-01
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| Series: | Structural Dynamics |
| Online Access: | http://dx.doi.org/10.1063/1.4974218 |