Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers.

The TSA Opal multiplex immunohistochemistry (mIHC) protocol (PerkinElmer) has been used to characterize immune infiltration in human cancers. This technique allows multiple biomarkers to be simultaneously stained in a single tissue section, which helps to elucidate the spatial relationship among ind...

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Bibliographic Details
Main Authors: Zhaoyu Sun, Richard Nyberg, Yaping Wu, Brady Bernard, William L Redmond
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0247238