General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex
General anesthetics are commonly used in animal models to study how sensory signals are represented in the brain. Here, we used two-photon (2P) calcium activity imaging with cellular resolution to investigate how neuronal activity in layer 2/3 of the mouse barrel cortex is modified under the influen...
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doaj-d50dea0554f44c509e299284ca494df52020-11-25T00:53:13ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022016-04-011010.3389/fncel.2016.00064173566General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortexThomas eLissek0Thomas eLissek1Horst A Obenhaus2Desiree eDitzel3Takeharu eNagai4Atsushi eMiyawaki5Rolf eSprengel6Mazahir T Hasan7Mazahir T Hasan8Max Planck Institute for Medical ResearchUniversity of HeidelbergMax Planck Institute for Medical ResearchMax Planck Institute for Medical ResearchOsaka UniversityRIKENMax Planck Institute for Medical ResearchCharité-Universitätsmedizin-BerlinMax Planck Institute for Medical ResearchGeneral anesthetics are commonly used in animal models to study how sensory signals are represented in the brain. Here, we used two-photon (2P) calcium activity imaging with cellular resolution to investigate how neuronal activity in layer 2/3 of the mouse barrel cortex is modified under the influence of different concentrations of chemically distinct general anesthetics. Our results show that a high isoflurane dose induces synchrony in local neuronal networks and these cortical activity patterns closely resemble those observed in EEG recordings under deep anesthesia. Moreover, ketamine and urethane also induced similar activity patterns. While investigating the effects of deep isoflurane anesthesia on whisker and auditory evoked responses in the barrel cortex, we found that dedicated spatial regions for sensory signal processing become disrupted. We propose that our isoflurane-2P imaging paradigm can serve as an attractive model system to dissect cellular and molecular mechanisms that induce the anesthetic state, and it might also provide important insight into sleep-like brain states and consciousness.http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00064/fullAnestheticsCerebral CortexIsofluranegenetically encoded calcium indicatorstwo-photon imaging |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thomas eLissek Thomas eLissek Horst A Obenhaus Desiree eDitzel Takeharu eNagai Atsushi eMiyawaki Rolf eSprengel Mazahir T Hasan Mazahir T Hasan |
spellingShingle |
Thomas eLissek Thomas eLissek Horst A Obenhaus Desiree eDitzel Takeharu eNagai Atsushi eMiyawaki Rolf eSprengel Mazahir T Hasan Mazahir T Hasan General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex Frontiers in Cellular Neuroscience Anesthetics Cerebral Cortex Isoflurane genetically encoded calcium indicators two-photon imaging |
author_facet |
Thomas eLissek Thomas eLissek Horst A Obenhaus Desiree eDitzel Takeharu eNagai Atsushi eMiyawaki Rolf eSprengel Mazahir T Hasan Mazahir T Hasan |
author_sort |
Thomas eLissek |
title |
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex |
title_short |
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex |
title_full |
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex |
title_fullStr |
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex |
title_full_unstemmed |
General anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex |
title_sort |
general anesthetic conditions induce network synchrony and disrupt sensory processing in the cortex |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2016-04-01 |
description |
General anesthetics are commonly used in animal models to study how sensory signals are represented in the brain. Here, we used two-photon (2P) calcium activity imaging with cellular resolution to investigate how neuronal activity in layer 2/3 of the mouse barrel cortex is modified under the influence of different concentrations of chemically distinct general anesthetics. Our results show that a high isoflurane dose induces synchrony in local neuronal networks and these cortical activity patterns closely resemble those observed in EEG recordings under deep anesthesia. Moreover, ketamine and urethane also induced similar activity patterns. While investigating the effects of deep isoflurane anesthesia on whisker and auditory evoked responses in the barrel cortex, we found that dedicated spatial regions for sensory signal processing become disrupted. We propose that our isoflurane-2P imaging paradigm can serve as an attractive model system to dissect cellular and molecular mechanisms that induce the anesthetic state, and it might also provide important insight into sleep-like brain states and consciousness. |
topic |
Anesthetics Cerebral Cortex Isoflurane genetically encoded calcium indicators two-photon imaging |
url |
http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00064/full |
work_keys_str_mv |
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