Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo
Abstract Background Previous studies have demonstrated remarkable tropism of mesenchymal stem cells (MSCs) toward malignant gliomas, making these cells a potential vehicle for delivery of therapeutic agents to disseminated glioblastoma (GBM) cells. However, the potential contribution of MSCs to tumo...
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doaj-d51cc7f04ed247449dd4fd8f0b1cad492020-11-25T02:02:27ZengBMCStem Cell Research & Therapy1757-65122018-11-019111310.1186/s13287-018-1049-0Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivoLorena Favaro Pavon0Tatiana Tais Sibov1Andrea Vieira de Souza2Edgar Ferreira da Cruz3Suzana M. F. Malheiros4Francisco Romero Cabral5Jean Gabriel de Souza6Pamela Boufleur7Daniela Mara de Oliveira8Silvia Regina Caminada de Toledo9Luciana C. Marti10Jackeline Moraes Malheiros11Fernando F. Paiva12Alberto Tannús13Sérgio Mascarenhas de Oliveira14Ana Marisa Chudzinski-Tavassi15Manoel A. de Paiva Neto16Sérgio Cavalheiro17Department of Neurosurgery, Federal University of São PauloDepartment of Neurosurgery, Federal University of São PauloExperimental Research Center, Hospital Israelita Albert EinsteinDiscipline of Nephrology, Federal University of São PauloDepartment of Neurosurgery, Federal University of São PauloExperimental Research Center, Hospital Israelita Albert EinsteinLaboratory of Molecular Biology, Butantan InstituteLaboratory of Molecular Biology, Butantan InstituteDepartment of Genetics and Morphology, University of BrasíliaPediatric Oncology Institute, Grupo de Apoio ao Adolescente e à Criança com Câncer (GRAACC), Federal University of São PauloExperimental Research Center, Hospital Israelita Albert EinsteinSão Carlos Institute of Physics, São Paulo UniversitySão Carlos Institute of Physics, São Paulo UniversitySão Carlos Institute of Physics, São Paulo UniversitySão Carlos Institute of Physics, São Paulo UniversityLaboratory of Molecular Biology, Butantan InstituteDepartment of Neurosurgery, Federal University of São PauloDepartment of Neurosurgery, Federal University of São PauloAbstract Background Previous studies have demonstrated remarkable tropism of mesenchymal stem cells (MSCs) toward malignant gliomas, making these cells a potential vehicle for delivery of therapeutic agents to disseminated glioblastoma (GBM) cells. However, the potential contribution of MSCs to tumor progression is a matter of concern. It has been suggested that CD133+ GBM stem cells secrete a variety of chemokines, including monocytes chemoattractant protein-1 (MCP-1/CCL2) and stromal cell-derived factor-1(SDF-1/CXCL12), which could act in this tropism. However, the role in the modulation of this tropism of the subpopulation of CD133+ cells, which initiate GBM and the mechanisms underlying the tropism of MSCs to CD133+ GBM cells and their effects on tumor development, remains poorly defined. Methods/results We found that isolated and cultured MSCs (human umbilical cord blood MSCs) express CCR2 and CXCR4, the respective receptors for MCP-1/CCL2 and SDF-1/CXCL12, and demonstrated, in vitro, that MCP-1/CCL2 and SDF-1/CXC12, secreted by CD133+ GBM cells from primary cell cultures, induce the migration of MSCs. In addition, we confirmed that after in vivo GBM tumor establishment, by stereotaxic implantation of the CD133+ GBM cells labeled with Qdots (705 nm), MSCs labeled with multimodal iron oxide nanoparticles (MION) conjugated to rhodamine-B (Rh-B) (MION-Rh), infused by caudal vein, were able to cross the blood-brain barrier of the animal and migrate to the tumor region. Evaluation GBM tumors histology showed that groups that received MSC demonstrated tumor development, glial invasiveness, and detection of a high number of cycling cells. Conclusions Therefore, in this study, we validated the chemotactic effect of MCP-1/CCL2 and SDF-1/CXCL12 in mediating the migration of MSCs toward CD133+ GBM cells. However, we observed that, after infiltrating the tumor, MSCs promote tumor growth in vivo probably by release of exosomes. Thus, the use of these cells as a therapeutic carrier strategy to target GBM cells must be approached with caution.http://link.springer.com/article/10.1186/s13287-018-1049-0CD133+ cellsMSCsTropismChemokinesExperimental modelExosomes |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Lorena Favaro Pavon Tatiana Tais Sibov Andrea Vieira de Souza Edgar Ferreira da Cruz Suzana M. F. Malheiros Francisco Romero Cabral Jean Gabriel de Souza Pamela Boufleur Daniela Mara de Oliveira Silvia Regina Caminada de Toledo Luciana C. Marti Jackeline Moraes Malheiros Fernando F. Paiva Alberto Tannús Sérgio Mascarenhas de Oliveira Ana Marisa Chudzinski-Tavassi Manoel A. de Paiva Neto Sérgio Cavalheiro |
spellingShingle |
Lorena Favaro Pavon Tatiana Tais Sibov Andrea Vieira de Souza Edgar Ferreira da Cruz Suzana M. F. Malheiros Francisco Romero Cabral Jean Gabriel de Souza Pamela Boufleur Daniela Mara de Oliveira Silvia Regina Caminada de Toledo Luciana C. Marti Jackeline Moraes Malheiros Fernando F. Paiva Alberto Tannús Sérgio Mascarenhas de Oliveira Ana Marisa Chudzinski-Tavassi Manoel A. de Paiva Neto Sérgio Cavalheiro Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo Stem Cell Research & Therapy CD133+ cells MSCs Tropism Chemokines Experimental model Exosomes |
author_facet |
Lorena Favaro Pavon Tatiana Tais Sibov Andrea Vieira de Souza Edgar Ferreira da Cruz Suzana M. F. Malheiros Francisco Romero Cabral Jean Gabriel de Souza Pamela Boufleur Daniela Mara de Oliveira Silvia Regina Caminada de Toledo Luciana C. Marti Jackeline Moraes Malheiros Fernando F. Paiva Alberto Tannús Sérgio Mascarenhas de Oliveira Ana Marisa Chudzinski-Tavassi Manoel A. de Paiva Neto Sérgio Cavalheiro |
author_sort |
Lorena Favaro Pavon |
title |
Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo |
title_short |
Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo |
title_full |
Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo |
title_fullStr |
Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo |
title_full_unstemmed |
Tropism of mesenchymal stem cell toward CD133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo |
title_sort |
tropism of mesenchymal stem cell toward cd133+ stem cell of glioblastoma in vitro and promote tumor proliferation in vivo |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2018-11-01 |
description |
Abstract Background Previous studies have demonstrated remarkable tropism of mesenchymal stem cells (MSCs) toward malignant gliomas, making these cells a potential vehicle for delivery of therapeutic agents to disseminated glioblastoma (GBM) cells. However, the potential contribution of MSCs to tumor progression is a matter of concern. It has been suggested that CD133+ GBM stem cells secrete a variety of chemokines, including monocytes chemoattractant protein-1 (MCP-1/CCL2) and stromal cell-derived factor-1(SDF-1/CXCL12), which could act in this tropism. However, the role in the modulation of this tropism of the subpopulation of CD133+ cells, which initiate GBM and the mechanisms underlying the tropism of MSCs to CD133+ GBM cells and their effects on tumor development, remains poorly defined. Methods/results We found that isolated and cultured MSCs (human umbilical cord blood MSCs) express CCR2 and CXCR4, the respective receptors for MCP-1/CCL2 and SDF-1/CXCL12, and demonstrated, in vitro, that MCP-1/CCL2 and SDF-1/CXC12, secreted by CD133+ GBM cells from primary cell cultures, induce the migration of MSCs. In addition, we confirmed that after in vivo GBM tumor establishment, by stereotaxic implantation of the CD133+ GBM cells labeled with Qdots (705 nm), MSCs labeled with multimodal iron oxide nanoparticles (MION) conjugated to rhodamine-B (Rh-B) (MION-Rh), infused by caudal vein, were able to cross the blood-brain barrier of the animal and migrate to the tumor region. Evaluation GBM tumors histology showed that groups that received MSC demonstrated tumor development, glial invasiveness, and detection of a high number of cycling cells. Conclusions Therefore, in this study, we validated the chemotactic effect of MCP-1/CCL2 and SDF-1/CXCL12 in mediating the migration of MSCs toward CD133+ GBM cells. However, we observed that, after infiltrating the tumor, MSCs promote tumor growth in vivo probably by release of exosomes. Thus, the use of these cells as a therapeutic carrier strategy to target GBM cells must be approached with caution. |
topic |
CD133+ cells MSCs Tropism Chemokines Experimental model Exosomes |
url |
http://link.springer.com/article/10.1186/s13287-018-1049-0 |
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