Genetic transformation of bean callus via Agrobacterium- mediated DNA transfer
Callus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient method to obtain transient gene expression in callu...
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Format: | Article |
Language: | English |
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University of Debrecen
2003-10-01
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Series: | International Journal of Horticultural Science |
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Online Access: | https://ojs.lib.unideb.hu/IJHS/article/view/405 |