Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins

Screening antibody libraries is an important step in establishing recombinant monoclonal antibodies. The colony assay can identify positive clones without almost any false-positives; however, its antibody library is smaller than those used in other recombinant screening methods such as phage display...

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Bibliographic Details
Main Authors: Yoshiro Hanyu, Mieko Kato
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/25/12/2905