Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins
Screening antibody libraries is an important step in establishing recombinant monoclonal antibodies. The colony assay can identify positive clones without almost any false-positives; however, its antibody library is smaller than those used in other recombinant screening methods such as phage display...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-06-01
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Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/25/12/2905 |