Time-gated detection of protein-protein interactions with transcriptional readout

Transcriptional assays, such as yeast two-hybrid and TANGO, that convert transient protein-protein interactions (PPIs) into stable expression of transgenes are powerful tools for PPI discovery, screens, and analysis of cell populations. However, such assays often have high background and lose inform...

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Bibliographic Details
Main Authors: Min Woo Kim, Wenjing Wang, Mateo I Sanchez, Robert Coukos, Mark von Zastrow, Alice Y Ting
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-11-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/30233