New Thermophilic α/β Class Epoxide Hydrolases Found in Metagenomes From Hot Environments

New Thermophilic α/β Class Epoxide Hydrolases Found in Metagenomes From Hot Environments

Two novel epoxide hydrolases (EHs), Sibe-EH and CH65-EH, were identified in the metagenomes of samples collected in hot springs in Russia and China, respectively. The two α/β hydrolase superfamily fold enzymes were cloned, over-expressed in Escherichia coli, purified and characterized. The new EHs w...

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Bibliographic Details
Main Authors: Erica Elisa Ferrandi, Christopher Sayer, Simone Antonio De Rose, Elisa Guazzelli, Carlotta Marchesi, Vahid Saneei, Michail N. Isupov, Jennifer A. Littlechild, Daniela Monti
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
epoxide hydrolase
metagenomics
industrial biocatalysis
stereoselectivity
protein structure
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2018.00144/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2018.00144/full

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