Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized polypyrrole (Ppy). Hybridization was measured using a...

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Bibliographic Details
Main Authors: David Danley, Wanda Lyon, John Cooper, Jodi Wilmoth, Nina Yazvenko, Karl Maurer
Format: Article
Language:English
Published: MDPI AG 2010-08-01
Series:Sensors
Subjects:
CombiMatrix microarray
biosensor
electrochemical detection
DNA
hybridization
polypyrrole
Online Access:http://www.mdpi.com/1424-8220/10/8/7371/

Internet

http://www.mdpi.com/1424-8220/10/8/7371/

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