GPR120 is an important inflammatory regulator in the development of osteoarthritis
Abstract Background The aim of this study was to investigate the regulatory role of G-protein coupled receptor 120 (GPR120) in the development and progression of osteoarthritis (OA). Methods GPR120 knockout (KO) and wild-type (WT) mice were used to create an animal model of OA by means of anterior c...
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doaj-d61cb45aa592419ca5370d8a962c91ed2020-11-25T02:03:37ZengBMCArthritis Research & Therapy1478-63622018-08-0120111110.1186/s13075-018-1660-6GPR120 is an important inflammatory regulator in the development of osteoarthritisYuanfeng Chen0Dan Zhang1Ki Wai Ho2Sien Lin3Wade Chun-Wai Suen4Huantian Zhang5Zhengang Zha6Gang Li7Po Sing Leung8Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversitySchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong KongDepartment of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales HospitalDepartment of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales HospitalDepartment of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales HospitalInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, the First Affiliated Hospital, Jinan UniversityDepartment of Orthopaedics & Traumatology, Li Ka Shing Institute of Health Sciences and Lui Che Woo Institute of Innovative Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales HospitalSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong KongAbstract Background The aim of this study was to investigate the regulatory role of G-protein coupled receptor 120 (GPR120) in the development and progression of osteoarthritis (OA). Methods GPR120 knockout (KO) and wild-type (WT) mice were used to create an animal model of OA by means of anterior cruciate ligament transection (ACLT) surgery. The severity of OA was staged and evaluated by histological examination, microcomputed tomography scan and enzyme-linked immunosorbent assay (ELISA). The anti-inflammatory effects of the GPR120 agonist docosahexaenoic acid (DHA) on human chondrocytes were further evaluated by specific inflammatory markers. In addition, the healing progression of a skin defect model was determined with histological assays. Results The GPR120-KO mice displayed an accelerated development of OA after ACLT. The secondary inflammation, cartilage degeneration, and subchondral bone aberrant changes were significantly elevated in the early phase of OA in KO mice relative to those in WT mice. In addition, we found that GPR120 levels were downregulated in OA patients compared with control subjects, whereas GPR120 activation with DHA exhibited anti-inflammatory effects in primary human chondrocytes in vitro. Moreover, results from the skin defect model showed that GPR120 agonism with DHA enhanced wound repair in mice, as shown by the downregulation of the number of CD68+ cells. Conclusions Our study suggests that GPR120 is an important inflammatory mediator during the development of OA, and that it is a potential marker for the diagnosis of high-risk patients with OA.http://link.springer.com/article/10.1186/s13075-018-1660-6G-protein coupled receptorsPolyunsaturated fatty acidsProinflammatory mediatorsCartilageSubchondral boneSkin defect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuanfeng Chen Dan Zhang Ki Wai Ho Sien Lin Wade Chun-Wai Suen Huantian Zhang Zhengang Zha Gang Li Po Sing Leung |
spellingShingle |
Yuanfeng Chen Dan Zhang Ki Wai Ho Sien Lin Wade Chun-Wai Suen Huantian Zhang Zhengang Zha Gang Li Po Sing Leung GPR120 is an important inflammatory regulator in the development of osteoarthritis Arthritis Research & Therapy G-protein coupled receptors Polyunsaturated fatty acids Proinflammatory mediators Cartilage Subchondral bone Skin defect |
author_facet |
Yuanfeng Chen Dan Zhang Ki Wai Ho Sien Lin Wade Chun-Wai Suen Huantian Zhang Zhengang Zha Gang Li Po Sing Leung |
author_sort |
Yuanfeng Chen |
title |
GPR120 is an important inflammatory regulator in the development of osteoarthritis |
title_short |
GPR120 is an important inflammatory regulator in the development of osteoarthritis |
title_full |
GPR120 is an important inflammatory regulator in the development of osteoarthritis |
title_fullStr |
GPR120 is an important inflammatory regulator in the development of osteoarthritis |
title_full_unstemmed |
GPR120 is an important inflammatory regulator in the development of osteoarthritis |
title_sort |
gpr120 is an important inflammatory regulator in the development of osteoarthritis |
publisher |
BMC |
series |
Arthritis Research & Therapy |
issn |
1478-6362 |
publishDate |
2018-08-01 |
description |
Abstract Background The aim of this study was to investigate the regulatory role of G-protein coupled receptor 120 (GPR120) in the development and progression of osteoarthritis (OA). Methods GPR120 knockout (KO) and wild-type (WT) mice were used to create an animal model of OA by means of anterior cruciate ligament transection (ACLT) surgery. The severity of OA was staged and evaluated by histological examination, microcomputed tomography scan and enzyme-linked immunosorbent assay (ELISA). The anti-inflammatory effects of the GPR120 agonist docosahexaenoic acid (DHA) on human chondrocytes were further evaluated by specific inflammatory markers. In addition, the healing progression of a skin defect model was determined with histological assays. Results The GPR120-KO mice displayed an accelerated development of OA after ACLT. The secondary inflammation, cartilage degeneration, and subchondral bone aberrant changes were significantly elevated in the early phase of OA in KO mice relative to those in WT mice. In addition, we found that GPR120 levels were downregulated in OA patients compared with control subjects, whereas GPR120 activation with DHA exhibited anti-inflammatory effects in primary human chondrocytes in vitro. Moreover, results from the skin defect model showed that GPR120 agonism with DHA enhanced wound repair in mice, as shown by the downregulation of the number of CD68+ cells. Conclusions Our study suggests that GPR120 is an important inflammatory mediator during the development of OA, and that it is a potential marker for the diagnosis of high-risk patients with OA. |
topic |
G-protein coupled receptors Polyunsaturated fatty acids Proinflammatory mediators Cartilage Subchondral bone Skin defect |
url |
http://link.springer.com/article/10.1186/s13075-018-1660-6 |
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