A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction

A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction

Abstract Background Single guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, the rules governing selection are not well established. Results We developed a facile transient assay to screen sgRNA efficiency. We then used it to test...

Full description

Bibliographic Details
Main Authors: Yan Liang, Aymerick Eudes, Sasha Yogiswara, Beibei Jing, Veronica T. Benites, Reo Yamanaka, Clarabelle Cheng-Yue, Edward E. Baidoo, Jenny C. Mortimer, Henrik V. Scheller, Dominique Loqué
Format: Article
Language:English
Published: BMC 2019-05-01
Series:Biotechnology for Biofuels
Subjects:
CRISPR/Cas9
Guide RNA efficiency
sgRNA
Constrained editing
HCT
GONST2
Online Access:http://link.springer.com/article/10.1186/s13068-019-1467-y

Internet

http://link.springer.com/article/10.1186/s13068-019-1467-y

Similar Items