Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

XylE is a bacterial xylose transporter and homologue of human glucose transporters GLUTs 1-4. HDX-MS, mutagenesis and MD simulations suggest that protonation of a conserved aspartate triggers conformational transition from outward- to inward facing state only in the presence of substrate xylose. In...

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Bibliographic Details
Main Authors: Ruyu Jia, Chloe Martens, Mrinal Shekhar, Shashank Pant, Grant A. Pellowe, Andy M. Lau, Heather E. Findlay, Nicola J. Harris, Emad Tajkhorshid, Paula J. Booth, Argyris Politis
Format: Article
Language:English
Published: Nature Publishing Group 2020-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20032-3

Internet

https://doi.org/10.1038/s41467-020-20032-3

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