High-Throughput Sequencing of Phage Display Libraries Reveals Parasitic Enrichment of Indel Mutants Caused by Amplification Bias

High-Throughput Sequencing of Phage Display Libraries Reveals Parasitic Enrichment of Indel Mutants Caused by Amplification Bias

The combination of phage display technology with high-throughput sequencing enables in-depth analysis of library diversity and selection-driven dynamics. We applied short-read sequencing of the mutagenized region on focused display libraries of two homologous nucleic acid modification eraser protein...

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Bibliographic Details
Main Authors: Sander Plessers, Vincent Van Deuren, Rob Lavigne, Johan Robben
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
phage display
AlkB
FTO
Illumina sequencing
Oxford nanopore sequencing
parasitic enrichment
Online Access:https://www.mdpi.com/1422-0067/22/11/5513

Internet

https://www.mdpi.com/1422-0067/22/11/5513

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