DNA EXTRACTION FROM HEAVY OIL CONTAMINATED MICROCOSMS AND RPOB GENE PCR AMPLIFICATION EXTRACCIÓN DE ADN A PARTIR DE MICROCOSMOS CONTAMINADOS CON PETRÓLEO PESADO Y AMPLIFICACIÓN DEL GEN RPOB

DNA EXTRACTION FROM HEAVY OIL CONTAMINATED MICROCOSMS AND RPOB GENE PCR AMPLIFICATION EXTRACCIÓN DE ADN A PARTIR DE MICROCOSMOS CONTAMINADOS CON PETRÓLEO PESADO Y AMPLIFICACIÓN DEL GEN RPOB

This work describes a new method for extracting genomic DNA from heavy oil-contaminated soils. This method combines soil washes using three washing solutions with enzymatic lysis (SW-EL method). The suitability of the SW-EL method was validated by extracting DNA from samples as small as 0.5 g from m...

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Bibliographic Details
Main Authors: Lucía C. Lozano-A., María A. Bautista, Jenny Dussan-G., Martha J. Vives-Flórez
Format: Article
Language:English
Published: Universidad de Antioquia 2008-01-01
Series:Actualidades Biológicas
Subjects:
extracción de ADN
amplificación PCR
microcosmos
petróleo pesado
rpoB
DNA extraction
heavy oil
microcosms
PCR amplification
Online Access:http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0304-35842008000100002

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http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0304-35842008000100002

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