Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis
The aim of this study was to identify a reliable ratio of omega-6/omega-3 fatty acids for regulating monocyte immunomodulation linked with atherosclerosis. We evaluated monocyte behavior on treatment with various dietary fatty oils with increasing fatty acid ratios (ω6:ω3): canola oil (ω6,5.6:ω3,1),...
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doaj-d74fae42627546069cc1c5b8dc03af8b2021-04-30T07:12:32ZengElsevierJournal of Functional Foods1756-46462018-02-0141258267Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosisAli A. Alshatwi0P. Subash-Babu1Corresponding author.; Adipogenesis and Immunobiology Research Lab, Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaAdipogenesis and Immunobiology Research Lab, Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaThe aim of this study was to identify a reliable ratio of omega-6/omega-3 fatty acids for regulating monocyte immunomodulation linked with atherosclerosis. We evaluated monocyte behavior on treatment with various dietary fatty oils with increasing fatty acid ratios (ω6:ω3): canola oil (ω6,5.6:ω3,1), olive oil (ω6,13.4:ω3,1), corn oil (ω6,52:ω3,1), coconut oil (saturated), and fish oil (ω6,1:ω3,2). Oxidized LDL-treated monocytes were treated with 0.5% and 2% oil emulsions (v/v) for 0, 2, 4, and 8 h. Oil red ‘O’ and Nile red staining demonstrated 70% and 30% lipid accumulation only in coconut and corn oil. The levels of IL-1β, IL-12β, IL-6, macrophage colony stimulation factors (IFNγ, IKBKγ1, IKK), and atherosclerosis-related immunomodulatory genes (TLR-4, TGFBR2, and IL33) were significantly increased after 8 h only in corn and coconut oil. Canola (5.6:1) and fish (2:1) oils are beneficial for immunoregulation and suppress macrophage-induced foam cell formation and atherosclerosis development.http://www.sciencedirect.com/science/article/pii/S1756464617307430Omega 6 and omega 3 fatty acidsVegetable oilMacrophageImmunomodulationAtherosclerosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali A. Alshatwi P. Subash-Babu |
spellingShingle |
Ali A. Alshatwi P. Subash-Babu Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis Journal of Functional Foods Omega 6 and omega 3 fatty acids Vegetable oil Macrophage Immunomodulation Atherosclerosis |
author_facet |
Ali A. Alshatwi P. Subash-Babu |
author_sort |
Ali A. Alshatwi |
title |
Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis |
title_short |
Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis |
title_full |
Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis |
title_fullStr |
Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis |
title_full_unstemmed |
Effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis |
title_sort |
effects of increasing ratios of dietary omega-6/omega-3 fatty acids on human monocyte immunomodulation linked with atherosclerosis |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2018-02-01 |
description |
The aim of this study was to identify a reliable ratio of omega-6/omega-3 fatty acids for regulating monocyte immunomodulation linked with atherosclerosis. We evaluated monocyte behavior on treatment with various dietary fatty oils with increasing fatty acid ratios (ω6:ω3): canola oil (ω6,5.6:ω3,1), olive oil (ω6,13.4:ω3,1), corn oil (ω6,52:ω3,1), coconut oil (saturated), and fish oil (ω6,1:ω3,2). Oxidized LDL-treated monocytes were treated with 0.5% and 2% oil emulsions (v/v) for 0, 2, 4, and 8 h. Oil red ‘O’ and Nile red staining demonstrated 70% and 30% lipid accumulation only in coconut and corn oil. The levels of IL-1β, IL-12β, IL-6, macrophage colony stimulation factors (IFNγ, IKBKγ1, IKK), and atherosclerosis-related immunomodulatory genes (TLR-4, TGFBR2, and IL33) were significantly increased after 8 h only in corn and coconut oil. Canola (5.6:1) and fish (2:1) oils are beneficial for immunoregulation and suppress macrophage-induced foam cell formation and atherosclerosis development. |
topic |
Omega 6 and omega 3 fatty acids Vegetable oil Macrophage Immunomodulation Atherosclerosis |
url |
http://www.sciencedirect.com/science/article/pii/S1756464617307430 |
work_keys_str_mv |
AT aliaalshatwi effectsofincreasingratiosofdietaryomega6omega3fattyacidsonhumanmonocyteimmunomodulationlinkedwithatherosclerosis AT psubashbabu effectsofincreasingratiosofdietaryomega6omega3fattyacidsonhumanmonocyteimmunomodulationlinkedwithatherosclerosis |
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