A gnotobiotic growth assay for Arabidopsis root microbiota reconstitution under iron limitation

Summary: We present a gnotobiotic system for microbiota reconstitution on Arabidopsis thaliana under contrasting iron availability. This system induces iron starvation in plants by providing an unavailable form, mimicking conditions in alkaline soils. Inoculation of taxonomically diverse bacteria re...

Full description

Bibliographic Details
Main Authors: Christopher J. Harbort, Masayoshi Hashimoto, Haruhiko Inoue, Paul Schulze-Lefert
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302136