MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state.
We report that a single growth factor, NM23-H1, enables serial passaging of both human ES and iPS cells in the absence of feeder cells, their conditioned media or bFGF in a fully defined xeno-free media on a novel defined, xeno-free surface. Stem cells cultured in this system show a gene expression...
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doaj-d7a6a9717e534841b4170f81b4ab8c112020-11-24T21:52:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5860110.1371/journal.pone.0058601MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state.Benoit J SmaggheAndrew K StewartMark G CarterLaura M SheltonKyle J BernierEric J HartmanAmy K CalhounVasilios M HatziioannouGabriele LillacciBrian A KirkBrian A DiNardoKenneth S KosikCynthia BamdadWe report that a single growth factor, NM23-H1, enables serial passaging of both human ES and iPS cells in the absence of feeder cells, their conditioned media or bFGF in a fully defined xeno-free media on a novel defined, xeno-free surface. Stem cells cultured in this system show a gene expression pattern indicative of a more "naïve" state than stem cells grown in bFGF-based media. NM23-H1 and MUC1* growth factor receptor cooperate to control stem cell self-replication. By manipulating the multimerization state of NM23-H1, we override the stem cell's inherent programming that turns off pluripotency and trick the cells into continuously replicating as pluripotent stem cells. Dimeric NM23-H1 binds to and dimerizes the extra cellular domain of the MUC1* transmembrane receptor which stimulates growth and promotes pluripotency. Inhibition of the NM23-H1/MUC1* interaction accelerates differentiation and causes a spike in miR-145 expression which signals a cell's exit from pluripotency.http://europepmc.org/articles/PMC3591366?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Benoit J Smagghe Andrew K Stewart Mark G Carter Laura M Shelton Kyle J Bernier Eric J Hartman Amy K Calhoun Vasilios M Hatziioannou Gabriele Lillacci Brian A Kirk Brian A DiNardo Kenneth S Kosik Cynthia Bamdad |
spellingShingle |
Benoit J Smagghe Andrew K Stewart Mark G Carter Laura M Shelton Kyle J Bernier Eric J Hartman Amy K Calhoun Vasilios M Hatziioannou Gabriele Lillacci Brian A Kirk Brian A DiNardo Kenneth S Kosik Cynthia Bamdad MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. PLoS ONE |
author_facet |
Benoit J Smagghe Andrew K Stewart Mark G Carter Laura M Shelton Kyle J Bernier Eric J Hartman Amy K Calhoun Vasilios M Hatziioannou Gabriele Lillacci Brian A Kirk Brian A DiNardo Kenneth S Kosik Cynthia Bamdad |
author_sort |
Benoit J Smagghe |
title |
MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. |
title_short |
MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. |
title_full |
MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. |
title_fullStr |
MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. |
title_full_unstemmed |
MUC1* ligand, NM23-H1, is a novel growth factor that maintains human stem cells in a more naïve state. |
title_sort |
muc1* ligand, nm23-h1, is a novel growth factor that maintains human stem cells in a more naïve state. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
We report that a single growth factor, NM23-H1, enables serial passaging of both human ES and iPS cells in the absence of feeder cells, their conditioned media or bFGF in a fully defined xeno-free media on a novel defined, xeno-free surface. Stem cells cultured in this system show a gene expression pattern indicative of a more "naïve" state than stem cells grown in bFGF-based media. NM23-H1 and MUC1* growth factor receptor cooperate to control stem cell self-replication. By manipulating the multimerization state of NM23-H1, we override the stem cell's inherent programming that turns off pluripotency and trick the cells into continuously replicating as pluripotent stem cells. Dimeric NM23-H1 binds to and dimerizes the extra cellular domain of the MUC1* transmembrane receptor which stimulates growth and promotes pluripotency. Inhibition of the NM23-H1/MUC1* interaction accelerates differentiation and causes a spike in miR-145 expression which signals a cell's exit from pluripotency. |
url |
http://europepmc.org/articles/PMC3591366?pdf=render |
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