Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety
A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selecti...
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| Format: | Article |
| Language: | English |
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Elsevier
1970-03-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520430056 |
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Article |
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doaj-d7b0bfb908984c1f9e8d85720ebacdfe2021-04-24T05:55:01ZengElsevierJournal of Lipid Research0022-22751970-03-01112144149Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moietyE.H. KolodnyR.O. BradyJ.M. QuirkJ.N. KanferA procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver beta-galactosidase to Tay-Sachs ganglioside-(3)H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule.http://www.sciencedirect.com/science/article/pii/S0022227520430056 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
E.H. Kolodny R.O. Brady J.M. Quirk J.N. Kanfer |
| spellingShingle |
E.H. Kolodny R.O. Brady J.M. Quirk J.N. Kanfer Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety Journal of Lipid Research |
| author_facet |
E.H. Kolodny R.O. Brady J.M. Quirk J.N. Kanfer |
| author_sort |
E.H. Kolodny |
| title |
Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety |
| title_short |
Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety |
| title_full |
Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety |
| title_fullStr |
Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety |
| title_full_unstemmed |
Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety |
| title_sort |
preparation of radioactive tay-sachs ganglioside labeled in the sialic acid moiety |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1970-03-01 |
| description |
A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver beta-galactosidase to Tay-Sachs ganglioside-(3)H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule. |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520430056 |
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