Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections.
BACKGROUND: Influenza A (flu) virus causes significant morbidity and mortality worldwide, and current vaccines require annual updating to protect against the rapidly arising antigenic variations due to antigenic shift and drift. In fact, current subunit or split flu vaccines rely exclusively on anti...
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doaj-d7df0ff886154443a74a919693951cdf2020-11-24T21:55:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0144e533610.1371/journal.pone.0005336Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections.Mohammed AlsharifiYoichi FuruyaTimothy R BowdenMario LobigsAulikki KoskinenMatthias RegnerLee TrinidadDavid B BoyleArno MüllbacherBACKGROUND: Influenza A (flu) virus causes significant morbidity and mortality worldwide, and current vaccines require annual updating to protect against the rapidly arising antigenic variations due to antigenic shift and drift. In fact, current subunit or split flu vaccines rely exclusively on antibody responses for protection and do not induce cytotoxic T (Tc) cell responses, which are broadly cross-reactive between virus strains. We have previously reported that gamma-ray inactivated flu virus can induce cross-reactive Tc cell responses. METHODOLOGY/PRINCIPAL FINDING: Here, we report that intranasal administration of purified gamma-ray inactivated human influenza A virus preparations (gamma-Flu) effectively induces heterotypic and cross-protective immunity. A single intranasal administration of gamma-A/PR8[H1N1] protects mice against lethal H5N1 and other heterotypic infections. CONCLUSIONS/SIGNIFICANCE: Intranasal gamma-Flu represents a unique approach for a cross-protective vaccine against both seasonal as well as possible future pandemic influenza A virus infections.http://europepmc.org/articles/PMC2671162?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammed Alsharifi Yoichi Furuya Timothy R Bowden Mario Lobigs Aulikki Koskinen Matthias Regner Lee Trinidad David B Boyle Arno Müllbacher |
spellingShingle |
Mohammed Alsharifi Yoichi Furuya Timothy R Bowden Mario Lobigs Aulikki Koskinen Matthias Regner Lee Trinidad David B Boyle Arno Müllbacher Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. PLoS ONE |
author_facet |
Mohammed Alsharifi Yoichi Furuya Timothy R Bowden Mario Lobigs Aulikki Koskinen Matthias Regner Lee Trinidad David B Boyle Arno Müllbacher |
author_sort |
Mohammed Alsharifi |
title |
Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. |
title_short |
Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. |
title_full |
Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. |
title_fullStr |
Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. |
title_full_unstemmed |
Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections. |
title_sort |
intranasal flu vaccine protective against seasonal and h5n1 avian influenza infections. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2009-01-01 |
description |
BACKGROUND: Influenza A (flu) virus causes significant morbidity and mortality worldwide, and current vaccines require annual updating to protect against the rapidly arising antigenic variations due to antigenic shift and drift. In fact, current subunit or split flu vaccines rely exclusively on antibody responses for protection and do not induce cytotoxic T (Tc) cell responses, which are broadly cross-reactive between virus strains. We have previously reported that gamma-ray inactivated flu virus can induce cross-reactive Tc cell responses. METHODOLOGY/PRINCIPAL FINDING: Here, we report that intranasal administration of purified gamma-ray inactivated human influenza A virus preparations (gamma-Flu) effectively induces heterotypic and cross-protective immunity. A single intranasal administration of gamma-A/PR8[H1N1] protects mice against lethal H5N1 and other heterotypic infections. CONCLUSIONS/SIGNIFICANCE: Intranasal gamma-Flu represents a unique approach for a cross-protective vaccine against both seasonal as well as possible future pandemic influenza A virus infections. |
url |
http://europepmc.org/articles/PMC2671162?pdf=render |
work_keys_str_mv |
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