Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis

Mycobacterium tuberculosis (Mtb) can enter the body through multiple routes, including via specialized transcytotic cells called microfold cells (M cell). However, the mechanistic basis for M cell entry remains undefined. Here, we show that M cell transcytosis depends on the Mtb Type VII secretion m...

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Main Authors:	Haaris S Khan, Vidhya R Nair, Cody R Ruhl, Samuel Alvarez-Arguedas, Jorge L Galvan Rendiz, Luis H Franco, Linzhang Huang, Philip W Shaul, Jiwoong Kim, Yang Xie, Ron B Mitchell, Michael U Shiloh
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2020-03-01
Series:	eLife
Subjects:	microfold cell mucosal immunology Mycobacterium tuberculosis scavenger receptor Type VII secretion system EsxA
Online Access:	https://elifesciences.org/articles/52551

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spelling	doaj-d7ed44f0af13427d8e7d84d1abe3eaa62021-05-05T20:53:18ZengeLife Sciences Publications LtdeLife2050-084X2020-03-01910.7554/eLife.52551Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosisHaaris S Khan0Vidhya R Nair1Cody R Ruhl2Samuel Alvarez-Arguedas3Jorge L Galvan Rendiz4Luis H Franco5Linzhang Huang6Philip W Shaul7Jiwoong Kim8Yang Xie9Ron B Mitchell10Michael U Shiloh11https://orcid.org/0000-0003-4329-2253Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United StatesCenter for Pulmonary and Vascular Biology, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, United StatesCenter for Pulmonary and Vascular Biology, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, United StatesQuantitative Biomedical Research Center, Department of Population and Data Sciences, University of Texas Southwestern Medical Center, Dallas, United StatesQuantitative Biomedical Research Center, Department of Population and Data Sciences, University of Texas Southwestern Medical Center, Dallas, United States; Harold C Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, United States; Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Otolaryngology, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States; Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, United StatesMycobacterium tuberculosis (Mtb) can enter the body through multiple routes, including via specialized transcytotic cells called microfold cells (M cell). However, the mechanistic basis for M cell entry remains undefined. Here, we show that M cell transcytosis depends on the Mtb Type VII secretion machine and its major virulence factor EsxA. We identify scavenger receptor B1 (SR-B1) as an EsxA receptor on airway M cells. SR-B1 is required for Mtb binding to and translocation across M cells in mouse and human tissue. Together, our data demonstrate a previously undescribed role for Mtb EsxA in mucosal invasion and identify SR-B1 as the airway M cell receptor for Mtb.https://elifesciences.org/articles/52551microfold cellmucosal immunologyMycobacterium tuberculosisscavenger receptorType VII secretion systemEsxA
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Haaris S Khan Vidhya R Nair Cody R Ruhl Samuel Alvarez-Arguedas Jorge L Galvan Rendiz Luis H Franco Linzhang Huang Philip W Shaul Jiwoong Kim Yang Xie Ron B Mitchell Michael U Shiloh
spellingShingle	Haaris S Khan Vidhya R Nair Cody R Ruhl Samuel Alvarez-Arguedas Jorge L Galvan Rendiz Luis H Franco Linzhang Huang Philip W Shaul Jiwoong Kim Yang Xie Ron B Mitchell Michael U Shiloh Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis eLife microfold cell mucosal immunology Mycobacterium tuberculosis scavenger receptor Type VII secretion system EsxA
author_facet	Haaris S Khan Vidhya R Nair Cody R Ruhl Samuel Alvarez-Arguedas Jorge L Galvan Rendiz Luis H Franco Linzhang Huang Philip W Shaul Jiwoong Kim Yang Xie Ron B Mitchell Michael U Shiloh
author_sort	Haaris S Khan
title	Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis
title_short	Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis
title_full	Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis
title_fullStr	Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis
title_full_unstemmed	Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis
title_sort	identification of scavenger receptor b1 as the airway microfold cell receptor for mycobacterium tuberculosis
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2020-03-01
description	Mycobacterium tuberculosis (Mtb) can enter the body through multiple routes, including via specialized transcytotic cells called microfold cells (M cell). However, the mechanistic basis for M cell entry remains undefined. Here, we show that M cell transcytosis depends on the Mtb Type VII secretion machine and its major virulence factor EsxA. We identify scavenger receptor B1 (SR-B1) as an EsxA receptor on airway M cells. SR-B1 is required for Mtb binding to and translocation across M cells in mouse and human tissue. Together, our data demonstrate a previously undescribed role for Mtb EsxA in mucosal invasion and identify SR-B1 as the airway M cell receptor for Mtb.
topic	microfold cell mucosal immunology Mycobacterium tuberculosis scavenger receptor Type VII secretion system EsxA
url	https://elifesciences.org/articles/52551
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Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis

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