Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development

Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development

As a critical and high-value tool to study the development of rumen, we established a stable rumen epithelial primary cell (REPC) culture from a two-week-old Holstein bull calf rumen epithelial tissue. The transcriptomic profiling of the REPC and the direct effects of butyrate on gene expression wer...

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Bibliographic Details
Main Authors: Shudai Lin, Lingzhao Fang, Xiaolong Kang, Shuli Liu, Mei Liu, Erin E. Connor, Ransom L. Baldwin, VI, George Liu, Cong-Jun Li
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Heliyon
Subjects:
Agriculture
Bioinformatics
Cell biology
Developmental biology
Butyrate
Epigenetic regulation
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844020309567

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http://www.sciencedirect.com/science/article/pii/S2405844020309567

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