Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of the technology, efficient and direct intracellular delivery of Cas9 ribonucleoprotein (RNP) complexes in...

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Bibliographic Details
Main Authors: Laurens Raes, Melissa Pille, Aranit Harizaj, Glenn Goetgeluk, Jelter Van Hoeck, Stephan Stremersch, Juan C. Fraire, Toon Brans, Olivier Gerrit de Jong, Roel Maas-Bakker, Enrico Mastrobattista, Pieter Vader, Stefaan C. De Smedt, Bart Vandekerckhove, Koen Raemdonck, Kevin Braeckmans
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
CRISPR-Cas9
gene editing
T cells
stem cells
photoporation
intracellular delivery
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253121002080

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http://www.sciencedirect.com/science/article/pii/S2162253121002080

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