A Heminested Polymerase Chain Reaction for the Detection of Brazilian Rabies Isolates from Vampire Bats and Herbivores

A heminested-PCR (hn-PCR) using primers to the nucleoprotein-coding gene in a nested set was evaluated in the detection of Brazilian strains of rabies virus (RV). A representative number of RV nucleoprotein sequences belonging to genotype 1 were aligned. Based on such alignment, primers were directe...

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Bibliographic Details
Main Authors: RM Soares, F Bernardi, SM Sakamoto, MB Heinemann, A Cortez, LM Alves, AD Meyer, FH Ito, LJ Richtzenhain
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2002-01-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000100019