AFEAP cloning: a precise and efficient method for large DNA sequence assembly

AFEAP cloning: a precise and efficient method for large DNA sequence assembly

Abstract Background Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), whi...

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Bibliographic Details
Main Authors: Fanli Zeng, Jinping Zang, Suhua Zhang, Zhimin Hao, Jingao Dong, Yibin Lin
Format: Article
Language:English
Published: BMC 2017-11-01
Series:BMC Biotechnology
Subjects:
Synthetic biology
DNA assembly
Assembly of fragment ends after PCR
Multi-fragment assembly
Bacterial artificial chromosomes
Online Access:http://link.springer.com/article/10.1186/s12896-017-0394-x

Internet

http://link.springer.com/article/10.1186/s12896-017-0394-x

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