A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culture medium

A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culture medium

Abstract 5-Hydroxytryptamine (also known as 5-HT, serotonin) is one of the monoamine neurotransmitters which is distributed widely in plasma and brain of mammals and plays important roles in physiological manipulations. In the present method, we describe the development of a simple, efficient and ra...

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Bibliographic Details
Main Authors: Qiangqiang He, Maoru Li, Xuechun Wang, Zhenjiang Xia, Yuzhi Du, Yan Li, Lixin Wei, Jing Shang
Format: Article
Language:English
Published: BMC 2019-06-01
Series:BMC Chemistry
Subjects:
HPLC–UV
Bioanalytical method validation
Cell culture medium
5-Hydroxytryptamine
RIN-14B cells
Online Access:http://link.springer.com/article/10.1186/s13065-019-0591-x

Internet

http://link.springer.com/article/10.1186/s13065-019-0591-x

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