Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library
<p>Abstract</p> <p>Background</p> <p>Hexaploid wheat (<it>Triticum aestivum </it>L.) possesses a large genome that contains 1.6 × 10<sup>10 </sup>bp of DNA. Isolation of a large number of gene sequences from complex gene families with a high leve...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-05-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/8/18 |