Microbial community composition and diversity via 16S rRNA gene amplicons: evaluating the illumina platform.

As new sequencing technologies become cheaper and older ones disappear, laboratories switch vendors and platforms. Validating the new setups is a crucial part of conducting rigorous scientific research. Here we report on the reliability and biases of performing bacterial 16S rRNA gene amplicon paire...

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Bibliographic Details
Main Authors: Lucas Sinclair, Omneya Ahmed Osman, Stefan Bertilsson, Alexander Eiler
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4315398?pdf=render