Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae

• Main Authors: Michelle Lopes Ribeiro-Guimarães, Eliana Blini Marengo, Antonio Jorge Tempone, Julio Jablonski Amaral, Clécio F Klitzke, Erika K Xavier da Silveira, Fernanda Calheta Vieira Portaro, Maria Cristina Vidal Pessolani
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2009-12-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Mycobacterium leprae; HtrA2; protease; enzymatic activity; FRET peptides; pathogenesis
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000800010
• ISSN: 0074-0276; 1678-8060

Members of the high temperature requirement A (HtrA) family of chaperone proteases have been shown to play a role in bacterial pathogenesis. In a recent report, we demonstrated that the gene ML0176, which codes for a predicted HtrA-like protease, a gene conserved in other species of mycobacteria, is transcribed by Mycobacterium leprae in human leprosy lesions. In the present study, the recombinant ML0176 protein was produced and its enzymatic properties investigated. M. lepraerecombinant ML0176 was able to hydrolyse a variety of synthetic and natural peptides. Similar to other HtrA proteins, this enzyme displayed maximum proteolytic activity at temperatures above 40°C and was completely inactivated by aprotinin, a protease inhibitor with high selectivity for serine proteases. Finally, analysis of M. leprae ML0176 specificity suggested a broader cleavage preference than that of previously described HtrAs homologues. In summary, we have identified an HtrA-like protease in M. lepraethat may constitute a potential new target for the development of novel prophylactic and/or therapeutic strategies against mycobacterial infections.