Insect cell culture in reagent bottles

Growing insect cells with high air space in culture vessel is common from the early development of suspension cell culture. We believed and followed it with the hope that it allows sufficient air for optimal cell growth. However, we missed to identify how much air exactly cells need for its growth a...

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Bibliographic Details
Main Authors: S. Rieffel, S. Roest, J. Klopp, S. Carnal, S. Marti, B. Gerhartz, B. Shrestha
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:MethodsX
Subjects:
BV
Sf9
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016114000077