Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage
Acetaminophen (APAP)-induced acute liver failure (ALF) is a serious health problem in developed countries. N-acetyl-L-cysteine (NAC), the current therapy for APAP-induced ALF, is not always effective, and liver transplantation is often needed. Opuntia spp. fruits are an important source of nutrients...
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doaj-d997f98e582543c6bd6b3d2934e0c29f2020-11-24T21:49:11ZengMDPI AGNutrients2072-66432016-10-0181060710.3390/nu8100607nu8100607Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver DamageHerson Antonio González-Ponce0María Consolación Martínez-Saldaña1Ana Rosa Rincón-Sánchez2María Teresa Sumaya-Martínez3Manon Buist-Homan4Klaas Nico Faber5Han Moshage6Fernando Jaramillo-Juárez7Department of Physiology and Pharmacology, Basic Science Center, Universidad Autónomade Aguascalientes, Aguascalientes 20131, MexicoDepartment of Morphology, Basic Science Center, Universidad Autónoma de Aguascalientes, Aguascalientes 20131, MexicoDepartment of Physiology, University Center of Health Sciences, Universidad de Guadalajara, Guadalajara 44340, MexicoFood Technology Unit, Secretary of Research and Graduate Studies, Universidad Autónoma de Nayarit, Tepic 63160, MexicoDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen 9713 GZ, The NetherlandsDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen 9713 GZ, The NetherlandsDepartment of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, Groningen 9713 GZ, The NetherlandsDepartment of Physiology and Pharmacology, Basic Science Center, Universidad Autónomade Aguascalientes, Aguascalientes 20131, MexicoAcetaminophen (APAP)-induced acute liver failure (ALF) is a serious health problem in developed countries. N-acetyl-L-cysteine (NAC), the current therapy for APAP-induced ALF, is not always effective, and liver transplantation is often needed. Opuntia spp. fruits are an important source of nutrients and contain high levels of bioactive compounds, including antioxidants. The aim of this study was to evaluate the hepatoprotective effect of Opuntia robusta and Opuntia streptacantha extracts against APAP-induced ALF. In addition, we analyzed the antioxidant activities of these extracts. Fruit extracts (800mg/kg/day, orally) were given prophylactically to male Wistar rats before intoxication with APAP (500 mg/kg, intraperitoneally). Rat hepatocyte cultures were exposed to 20mmol/LAPAP, and necrosis was assessed by LDH leakage. Opuntia robusta had significantly higher levels of antioxidants than Opuntia streptacantha. Both extracts significantly attenuated APAP-induced injury markers AST, ALT and ALP and improved liver histology. The Opuntia extracts reversed APAP-induced depletion of liver GSH and glycogen stores. In cultured hepatocytes, Opuntia extracts significantly reduced leakage of LDH and cell necrosis, both prophylactically and therapeutically. Both extracts appeared to be superior to NAC when used therapeutically. We conclude that Opuntia extracts are hepatoprotective and can be used as a nutraceutical to prevent ALF.http://www.mdpi.com/2072-6643/8/10/607Opuntia fruitsacetaminophenacute liver failureantioxidantshepatoprotectivenutraceutical |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Herson Antonio González-Ponce María Consolación Martínez-Saldaña Ana Rosa Rincón-Sánchez María Teresa Sumaya-Martínez Manon Buist-Homan Klaas Nico Faber Han Moshage Fernando Jaramillo-Juárez |
spellingShingle |
Herson Antonio González-Ponce María Consolación Martínez-Saldaña Ana Rosa Rincón-Sánchez María Teresa Sumaya-Martínez Manon Buist-Homan Klaas Nico Faber Han Moshage Fernando Jaramillo-Juárez Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage Nutrients Opuntia fruits acetaminophen acute liver failure antioxidants hepatoprotective nutraceutical |
author_facet |
Herson Antonio González-Ponce María Consolación Martínez-Saldaña Ana Rosa Rincón-Sánchez María Teresa Sumaya-Martínez Manon Buist-Homan Klaas Nico Faber Han Moshage Fernando Jaramillo-Juárez |
author_sort |
Herson Antonio González-Ponce |
title |
Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage |
title_short |
Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage |
title_full |
Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage |
title_fullStr |
Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage |
title_full_unstemmed |
Hepatoprotective Effect of Opuntia robusta and Opuntia streptacantha Fruits against Acetaminophen-Induced Acute Liver Damage |
title_sort |
hepatoprotective effect of opuntia robusta and opuntia streptacantha fruits against acetaminophen-induced acute liver damage |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2016-10-01 |
description |
Acetaminophen (APAP)-induced acute liver failure (ALF) is a serious health problem in developed countries. N-acetyl-L-cysteine (NAC), the current therapy for APAP-induced ALF, is not always effective, and liver transplantation is often needed. Opuntia spp. fruits are an important source of nutrients and contain high levels of bioactive compounds, including antioxidants. The aim of this study was to evaluate the hepatoprotective effect of Opuntia robusta and Opuntia streptacantha extracts against APAP-induced ALF. In addition, we analyzed the antioxidant activities of these extracts. Fruit extracts (800mg/kg/day, orally) were given prophylactically to male Wistar rats before intoxication with APAP (500 mg/kg, intraperitoneally). Rat hepatocyte cultures were exposed to 20mmol/LAPAP, and necrosis was assessed by LDH leakage. Opuntia robusta had significantly higher levels of antioxidants than Opuntia streptacantha. Both extracts significantly attenuated APAP-induced injury markers AST, ALT and ALP and improved liver histology. The Opuntia extracts reversed APAP-induced depletion of liver GSH and glycogen stores. In cultured hepatocytes, Opuntia extracts significantly reduced leakage of LDH and cell necrosis, both prophylactically and therapeutically. Both extracts appeared to be superior to NAC when used therapeutically. We conclude that Opuntia extracts are hepatoprotective and can be used as a nutraceutical to prevent ALF. |
topic |
Opuntia fruits acetaminophen acute liver failure antioxidants hepatoprotective nutraceutical |
url |
http://www.mdpi.com/2072-6643/8/10/607 |
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