Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study
The methylation status of the target promoter sequences of p15INK4B (p15) and O6-methylguanine-DNA methyltransferase (MGMT) genes was studied by methylation-specific PCR in 10 adult patients with de novo B-cell non- Hodgkin lymphoma (B-NHL). The aberrant hypermethylation of the p15 gene was more fre...
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University of Belgrade, University of Novi Sad
2010-01-01
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doaj-d9b78e496daf4259a4c009c087ccd95a2020-11-24T22:31:48ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642010-01-0162221122110.2298/ABS1002211KFrequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot studyKraguljac-Kurtović NadaKrajnović MilenaDimitrijević B.Mihaljević BiljanaGotić MirjanaKrtolica KoviljkaThe methylation status of the target promoter sequences of p15INK4B (p15) and O6-methylguanine-DNA methyltransferase (MGMT) genes was studied by methylation-specific PCR in 10 adult patients with de novo B-cell non- Hodgkin lymphoma (B-NHL). The aberrant hypermethylation of the p15 gene was more frequent (50%) compared to the hypermethylation of the MGMT gene (30%), and was detected in different types of B-NHL in both genes. Hypermethylation of the MGMT gene occurred exclusively in association with the hypermethylation of the p15 gene. All lymphoma patients with hypermethylation of the p15 and/or MGMT genes had a higher clinical stage of the disease (IV - V). We show the association of anemia and/or thrombocytopenia with the hypermethylation of the p15 gene, ascribing the p15 gene as a potential prognostic marker in B-NHL. Comethylation of MGMT with the p15 gene represents a novel finding and presents both genes as candidates for future studies of the hypermethylation profiles of B-NHL. http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641002211K.pdfMethylation statusp15INK4BO6-methylguanine-DNA methyltransferaseB-cell non-Hodgkin lymphoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kraguljac-Kurtović Nada Krajnović Milena Dimitrijević B. Mihaljević Biljana Gotić Mirjana Krtolica Koviljka |
spellingShingle |
Kraguljac-Kurtović Nada Krajnović Milena Dimitrijević B. Mihaljević Biljana Gotić Mirjana Krtolica Koviljka Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study Archives of Biological Sciences Methylation status p15INK4B O6-methylguanine-DNA methyltransferase B-cell non-Hodgkin lymphoma |
author_facet |
Kraguljac-Kurtović Nada Krajnović Milena Dimitrijević B. Mihaljević Biljana Gotić Mirjana Krtolica Koviljka |
author_sort |
Kraguljac-Kurtović Nada |
title |
Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study |
title_short |
Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study |
title_full |
Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study |
title_fullStr |
Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study |
title_full_unstemmed |
Frequency of aberrant promoter methylation of p15INK4b and O6-methylguanine-DNA methyltransferase genes in b-cell non-Hodgkin lymphoma: A pilot study |
title_sort |
frequency of aberrant promoter methylation of p15ink4b and o6-methylguanine-dna methyltransferase genes in b-cell non-hodgkin lymphoma: a pilot study |
publisher |
University of Belgrade, University of Novi Sad |
series |
Archives of Biological Sciences |
issn |
0354-4664 |
publishDate |
2010-01-01 |
description |
The methylation status of the target promoter sequences of p15INK4B (p15) and O6-methylguanine-DNA methyltransferase (MGMT) genes was studied by methylation-specific PCR in 10 adult patients with de novo B-cell non- Hodgkin lymphoma (B-NHL). The aberrant hypermethylation of the p15 gene was more frequent (50%) compared to the hypermethylation of the MGMT gene (30%), and was detected in different types of B-NHL in both genes. Hypermethylation of the MGMT gene occurred exclusively in association with the hypermethylation of the p15 gene. All lymphoma patients with hypermethylation of the p15 and/or MGMT genes had a higher clinical stage of the disease (IV - V). We show the association of anemia and/or thrombocytopenia with the hypermethylation of the p15 gene, ascribing the p15 gene as a potential prognostic marker in B-NHL. Comethylation of MGMT with the p15 gene represents a novel finding and presents both genes as candidates for future studies of the hypermethylation profiles of B-NHL. |
topic |
Methylation status p15INK4B O6-methylguanine-DNA methyltransferase B-cell non-Hodgkin lymphoma |
url |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641002211K.pdf |
work_keys_str_mv |
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