Optimization of ISSR-PCR reaction system and selection of primers in <i>Bryum argenteum</i>
In order to determine optimum ISSR-PCR reaction system for moss <i>Bryum argenteum</i>,the concentrations of template DNA primers,dNTPs,Mg<sup>2+</sup> and Taq DNA polymerase were optimized in four levels by PCR orthogonal experimental method. The appropriate primers were scr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals Center of Shanghai Normal University
2017-02-01
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| Series: | Journal of Shanghai Normal University (Natural Sciences) |
| Subjects: | |
| Online Access: | http://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/view_abstract.aspx?file_no=20170509&flag=1 |