Optimization of ISSR-PCR reaction system and selection of primers in <i>Bryum argenteum</i>

Optimization of ISSR-PCR reaction system and selection of primers in <i>Bryum argenteum</i>

In order to determine optimum ISSR-PCR reaction system for moss <i>Bryum argenteum</i>,the concentrations of template DNA primers,dNTPs,Mg<sup>2+</sup> and Taq DNA polymerase were optimized in four levels by PCR orthogonal experimental method. The appropriate primers were scr...

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Bibliographic Details
Main Authors:	Ma Xiaoying, Wei Wei, Zang Cheng, Yu Jing
Format:	Article
Language:	English
Published:	Academic Journals Center of Shanghai Normal University 2017-02-01
Series:	Journal of Shanghai Normal University (Natural Sciences)
Subjects:	<i>Bryum argenteum</i> ISSR orthogonal design primer screening
Online Access:	http://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/view_abstract.aspx?file_no=20170509&flag=1

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