Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens

Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens

Abstract Background Genome-wide loss-of-function screens using the CRISPR/Cas9 system allow the efficient discovery of cancer cell vulnerabilities. While several studies have focused on correcting for DNA cleavage toxicity biases associated with copy number alterations, the effects of sgRNAs co-targ...

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Bibliographic Details
Main Authors: Jean-Philippe Fortin, Jenille Tan, Karen E. Gascoigne, Peter M. Haverty, William F. Forrest, Michael R. Costa, Scott E. Martin
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Genome Biology
Subjects:
CRISPR/Cas9
Functional genomics
Gene essentiality
Cancer vulnerability
Synthetic lethality
Achilles
Online Access:http://link.springer.com/article/10.1186/s13059-019-1621-7

Internet

http://link.springer.com/article/10.1186/s13059-019-1621-7

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