AANAT transgenic sheep generated via OPS vitrified-microinjected pronuclear embryos and reproduction efficiency of the transgenic offspring

Background The open pulled straw (OPS) vitrification method has been successfully applied in mouse, pig, and goat embryos as well as in buffalo oocytes, but it has not yet been applied to the microinjected embryos. This study examined the effects of OPS vitrification on embryo development and the re...

Full description

Bibliographic Details
Main Authors: Xiuzhi Tian, Dongying Lv, Teng Ma, Shoulong Deng, Minghui Yang, Yukun Song, Xiaosheng Zhang, Jinglong Zhang, Juncai Fu, Zhengxing Lian, Shien Zhu, Yingjie Wu, Yiming Xing, Guoshi Liu
Format: Article
Language:English
Published: PeerJ Inc. 2018-08-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/5420.pdf