De novo assembly of highly polymorphic metagenomic data using in situ generated reference sequences and a novel BLAST-based assembly pipeline

De novo assembly of highly polymorphic metagenomic data using in situ generated reference sequences and a novel BLAST-based assembly pipeline

Abstract Background The accuracy of metagenomic assembly is usually compromised by high levels of polymorphism due to divergent reads from the same genomic region recognized as different loci when sequenced and assembled together. A viral quasispecies is a group of abundant and diversified genetical...

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Bibliographic Details
Main Authors: You-Yu Lin, Chia-Hung Hsieh, Jiun-Hong Chen, Xuemei Lu, Jia-Horng Kao, Pei-Jer Chen, Ding-Shinn Chen, Hurng-Yi Wang
Format: Article
Language:English
Published: BMC 2017-04-01
Series:BMC Bioinformatics
Subjects:
Next generation sequencing
Metagenomics
Hepatitis B virus
Sequence assembly
Assembly pipeline
Online Access:http://link.springer.com/article/10.1186/s12859-017-1630-z

Internet

http://link.springer.com/article/10.1186/s12859-017-1630-z

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