Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

Molecular Brightness Approach for FRET Analysis of Donor-Linker-Acceptor Constructs at the Single Molecule Level: A Concept

In this report, we have developed a simple approach using single-detector fluorescence autocorrelation spectroscopy (FCS) to investigate the Förster resonance energy transfer (FRET) of genetically encoded, freely diffusing crTC2.1 (mTurquoise2.1–linker–mCitrine) at the single molecule level. We hypo...

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Bibliographic Details
Main Authors: Taryn M. Kay, Cody P. Aplin, Rowan Simonet, Julie Beenken, Robert C. Miller, Christin Libal, Arnold J. Boersma, Erin D. Sheets, Ahmed A. Heikal
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Molecular Biosciences
Subjects:
FRET
FCS
donor-linker-acceptor
mTurquoise2.1
mCitrine
crTC2.1
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.730394/full

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https://www.frontiersin.org/articles/10.3389/fmolb.2021.730394/full

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