EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM Evaluación del factor de crecimiento IGF-I en la maduración in vitro de ovocitos de perras en el medio TCM 199 AVALIAÇÃO DO FATOR DE CRESCIMENTO IGF-I NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS NO MEIO TCM 199

The aim was to evaluate the supplementation of recombinant IGF-I growth factor at concentration of 100?g/mL in 500µL of TCM 199 medium in the in vitro maturation of canine oocytes. The oocytes were stained with isotiocianate of fluorescein (Lens cullinaris) and Hoeschst 33342 for nuclear and cytopla...

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Bibliographic Details
Main Authors: Maricy Apparício Ferreira, Ana Paula Coelho Ribeiro, Marco Antônio Machado, Kellen de Sousa, Oliveira, Gilson Hélio Toniollo
Format: Article
Language:English
Published: Universidade Federal de Goiás 2009-07-01
Series:Ciência Animal Brasileira
Subjects:
Online Access:http://www.revistas.ufg.br/index.php/vet/article/view/1121