CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters

CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters

Abstract Background Recent experimental efforts of CRISPR-Cas9 systems have shown that off-target binding and cleavage are a concern for the system and that this is highly dependent on the selected guide RNA (gRNA) design. Computational predictions of off-targets have been proposed as an attractive...

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Bibliographic Details
Main Authors: Ferhat Alkan, Anne Wenzel, Christian Anthon, Jakob Hull Havgaard, Jan Gorodkin
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Genome Biology
Subjects:
CRISPR-Cas9
Off-targets
Off-target scoring
Energy models
gRNA specificity
gRNA design
Online Access:http://link.springer.com/article/10.1186/s13059-018-1534-x

Internet

http://link.springer.com/article/10.1186/s13059-018-1534-x

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