Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation

Abstract Background The purification of peripheral blood mononuclear cells (PBMCs) by means of density gradient (1.07 g/mL) centrifugation is one of the most commonly used methods in diagnostics and research laboratories as well as in biobanks. Here, we evaluated whether it was possible to set up an...

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Bibliographic Details
Main Authors: Luigi Coppola, Giovanni Smaldone, Alessandra Cianflone, Simona Baselice, Peppino Mirabelli, Marco Salvatore
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Journal of Translational Medicine
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12967-019-2125-7